N.N. Boyko Ph.D. (Pharm.), Associate Professor, Department of Pharmaceutical Technology, Belgorod National Research E-mail:; ORCID: 0000-0001-9222-2935 D.I. Pisarev Dr.Sc. (Pharm.), Professor, Department of Chemistry, Belgorod National Research E-mail:; ORCID: 0000-0002-2996-7712 E.T. Zhilyakova Dr.Sc. (Pharm.), Professor, Head of Department of Pharmaceutical Technology, Belgorod National Research E-mail: A.Yu. Maljutina Ph.D. (Pharm.), Associate Professor, Department of Chemistry, Belgorod National Research E-mail: O.O. Novikov Dr.Sc. (Pharm.), Professor, Head of Test Department «Drugs quality control center» of the Shared Research and Education Center, Рeoples’ Friendship University of Russia (Moscow) E-mail:; ORCID: 0000-0003-3145-6783

Objective: when conducting forensic studies in the forensic chemistry department of the Krasnoyarsk Regional Bureau of Forensic Medicine, there were many cases of identification of nonsteroidal anti-inflammatory drugs in cadaveric material, such as Ibuprofen, Diclofenac, and others. Drugs found predominantly in individuals suspected of non-medical use of psychoactive substances can serve as specific markers of the latter. Earlier, Etoricoxib was not defined in expert material, therefore the aim of the presented research was to develop a method for determining this agent in cadaver blood. Materials and methods: cadaver blood was tested, a standard solution of Etoricoxib prepared from a commercial drug Costarox (Sandoz), tested for purity and content of the active substance, the qualification of reagents complied with the requirements for examination. The analysis was carried out on a «Milichrom A-02» liquid microcolumn chromatograph with a spectrophotometric detector of the UV-range, multiwave detection, with the MultiChrom processing program. The study was carried out in compliance with the requirements of evidence-based medicine. The reliability of the study is provided by the formation of control groups and comparisons, using the correct methods of statistical processing of results. Results. A method for determining the non-steroidal anti-inflammatory agent Etoricoxib by the method of gradient high-performance liquid chro-matography in cadaveric blood in relation to forensic chemical studies has been developed, ensuring a reduction in the duration and material costs of the analysis without compromising the evidence of the results. This effect is achieved by using as a mobile phase a mixture of eluents - acetonitrile (B) and lithium perchlorate (A) (gradient elution from 5 to 100% eluent B in eluent A), as well as the use of an internal standard (Anekain). The detection limit of etoricoxib was 2 ng/ml, the limit of quantitative determination was 6 ng/ml. The relative standard deviation of Sr does not exceed 0.08. The percentage of extraction of Etoricoxib from cadaveric blood ranged from 32.7 to 37.4%. Conclusions: The described method is characterized by a fairly high sensitivity, reproducibility, efficiency, express. Obtaining quantitative values of the concentration of Etoricoxib will allow to differentiate the purpose of the drug, to specify the cause of death. The described technique is used in the practice of the Krasnoyarsk Bureau of Forensic Medical Examination and can be recommended for routine forensic chemical studies in order to establish the presence and content of Etoricoxib in human cadaveric blood.

determination method
high performance liquid chromatography
forensic chemical study

  1. Prikaz Minzdravsotsrazvitija RF ot 12.05.2010 g. № 346n «Ob utverzhdenii Porjadka organizatsii i proizvodstva sudebno-meditsinskih ekspertiz v gosudarstvennyh sudebno-ekspertnyh uchrezhdenijah Rossijskoj Federatsii» (Prikaz Minzdravsocrazvitiya RF ot 12.05.2010 g. № 346n «Ob utverzhdenii Poryadka organizacii i proizvodstva sudebno-medicinskih ekspertiz v gosudarstvennyh sudebno-ekspertnyh uchrezhdeniyah Rossijskoj Federacii»).
  2. Moffat A.C., Osselton M. D., Widdop B. Clarke’s analysis of drugs and poisons in pharmaceuticals, body fluids and postmortem material / London: Pharmaceutical Press, 2011; 2473 p.
  3. Khade T.S., Saindane D.S., Pore Y.V. Simultaneous spectrophotometric determination of etoricoxib and paracetamol in a laboratory mixture // Int. J. PharmTech. Res. 2010; 2(1): 623–626.
  4. Kumar S. S., Natraj D., Khan A. et al. Development and validation of RP-HPLC method for simultaneous estimation of etoricoxib and thiocolchicoside in pharmaceutical dosage forms // IJRPC. 2011; 1(3): 649–653.
  5. Chandra R., Sanghi A., Kumar D., Hindwan K.K. Develop a simple RP-HPLC and UV-Visible method for estimation of etoricoxib from pharmaceutical dosage // BBB. 2014; 2(4): 706–713.
  6. Brautigam L., Nefflen J.U., Geisslinger G. Determination of etoricoxib in human plasma by liquid chromatography–tandem mass spectrometry with electrospray ionization // Journal of Chromatography B. 2003; 788(2): 309–15.
  7. Kumar V.V. P., Vinu M.C.A., Ramani A.V., Mullangi R., Srinivas N.R. Simultaneous quantitation of etoricoxib, salicylic acid, valdecoxib, ketoprofen, nimesulide and celecoxib in plasma by high-performance liquid chromatography with UV detection // Biomed. Chromatogr. 2006; 20(1): 125–132.
  8. Rao R.N., Meena S., Rao A.R. An overview of the recent developments in analytical methodologies for determination of COX-2 inhibitors in bulk drugs, pharmaceuticals and biological matrices // J. Pharm. Biomed. Anal. 2005; 39: 349–63.
  9. Ivanov R., Sekareva G., Kravtsova O., Kudlaj D., Luk'janov S., Tihonova I., Demin A., Maksumova L., Nikiti-
  10. na I., Obuhov A., Zajtsev D., Stepanov A., Nosyreva M., Samsonov M. Pravila provedenija issledovanij bioanalogovyh lekarstvennyh sredstv (bioanalogov) // Farmakokinetika i farmakodinamika. 2014. № 1. S. 21–36. (Ivanov R., Sekaryova G., Kravcova O., Kudlaj D., Luk’yanov S., Tihonova I., Dyomin A., Maksumova L., Nikitina I., Obuhov A., Zajcev D., Stepanov A., Nosyreva M., Samsonov M. Pravila provedeniya issledovanij bioanalogovyh lekarstvennyh sredstv (bioanalogov) // Farmakokinetika i farmakodinamika. 2014. № 1. S. 21–36).
  11. Mamaev A.N., Kudlaj D.A. Vizualizatsija dannyh v prezentatsijah, otchetah i issledovanijah. M. 2011. (Mama-
  12. ev A.N., Kudlaj D.A. Vizualizaciya dannyh v prezentaciyah, otchetah i issledovaniyah. M. 2011.).