PHYSIOLOGICAL CHARACTERISTICS SUSPENSION CULTURE OF RHODIOLA ROSEA L. AT CULTIVATION ON THE MODIFIED NUTRIENT MEDIUM

DOI: https://doi.org/10.29296/25877313-2019-05-05
Номер журнала: 
5
Год издания: 
2019

P.S. Savin Ph.D. (Biol.), Leading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: savin-pavel@list.ru T.A. Savina Ph.D. (Biol.), Head of Biotechnology Section, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow). E-mail: savina-tatyana57@yandex.ru S.B. Myasnikova Research Scientist, Biotechnology Section, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)

To use cell plant cultures in the production of biologically active substances, one must know their properties, including the patterns of growth and biosynthetic activity. As a result of the study of the physiological features of the Rhodíola rosea L. culture of the Rr(S) 2013VILAR strain in the dynamics of biomass accumulation under conditions of deep cultivation on a macrosolated nutrient medium, the duration of culture growth was determined to be 1520 days. The process of culture growth under conditions of deep cultivation can be described by a growth curve having an S-shape. The factor of physiological heterogeneity of the suspension culture is noted, which can significantly influence the final results on biomass accumulation and its bi-oproductivity. It was found that the maximum consumption of oxygen by the cell culture is 12•104 mol/l•h and falls on the 8th day of growth, when the cells are in the phase of regular growth and begin to actively share. To maintain the active growth of the suspension culture of Rhodíola rosea L. strain Rr(S) 2013 VILAR in the cultivation vessels, it is necessary to create a mass transfer coefficient of 8 h1. It is shown that the accumulation of the sum of phenylpropanoids in the cell culture of Rhodíola rosea L. of the Rr(S) 2013VILAR strain occurs in parallel with the growth of biomass.

Ключевые слова: 
cell culture of Rhodiola rosea (R. rosea)
suspension culture
nutrient medium
respiratory activity
phenylpropanoids

Список литературы: 
  1. Grech-Baran Marta, Sykłowska-Baranek Katarzyna, Pietrosiuk Agnieszka. Biotechnological approaches to enhance salidroside, rosin and its derivatives production in selected Rhodiola spp. in vitro cultures // Phytochem Rev. 2015. № 14. S. 657–674.
  2. Lupanova I.A., Strelkova L.B., Savina T.A., Ferubko E.V. Study of biological activity of the Rhodiola rosea L. biomass extract using specific enzymatic test systems in vitro // Scientific-practical journal «Issues of quality assurance of medicines». 2017. № 3 (17). P. 9–15.
  3. Savina T.A., Myasnikova S.B., Balakina M.V. Optim ization of conditions of cultivation for suspension culture of the Rhodiola rosea L. // Questions of biological, medical and pharmaceutical chemistry. 2016. №. 9. C. 13–18.
  4. Savina T.A., Myasnikova S.B., Savin P.S. The influence of the composition of the nutrient medium on the growth and biosynthetic parameters of suspension culture of Rhodiola rosea (Rhodiola rosea L.) // Questions of biological medical and pharmaceutical chemistry. M. 2018. № 8. P. 9–12.
  5. Lezhnev E.I., Popova I.I., Lavrovskaya V.P. Measurement error correction of Clark oxygen electrode Autoconsumption //Scientific instrument engineering. M. V. 18. №. 1. 2008. Р. 76–81.
  6. Vasil'ev N.N., Ambrosov V.A., Skladnev A.A. Modelirovanie processov mikrobiologicheskogo sinteza [Modelling of microbiological synthesis processes]. Moscow, Lesn. prom. Publ. 1975. 344 p.
  7. Tsybulko N., Savina T. A. Method for the quantitative determination of phenylpropanoids in cell culture Rhodiola rosea // From plant to drug: tradition and modernity: collection of scientific works. Tr. GNU VILAR. M. 2014. Р. 48–52.

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat или click here to download the PDF file.