ANALYSIS OF METABOLIC CHANGES IN LIVER MITOCHONDRIA AND RED BLOOD CELLS IN ESSENTIAL HYPERCHOLESTEROLEMIA IN RATS

DOI: https://doi.org/10.29296/25877313-2020-12-06
Issue: 
12
Year: 
2020

Z.I. Mikashinovich Dr.Sc. (Biol.), Professor, Department of General and Clinical Biochemistry № 1, Rostov State Medical University (Rostov-on-Don) I.A. Semenets Post-graduate Student, Department of General and Clinical Biochemistry № 1, Rostov State Medical University (Rostov-on-Don) E-mail: semenets.i.a@mail.ru A.V. Romashenko Post-graduate Student, Department of General and Clinical Biochemistry № 1, Rostov State Medical University (Rostov-on-Don)

Relevance. One of the main causes of increased mortality is vascular atherosclerosis against the background of hypercholesterolemia. The aim of the study was to analyze the biochemical changes occurring in liver cells and red blood cells in the simulation of essential hypercholesterolemia in rats. Material and methods. In liver mitochondrial homogenates and blood red blood cells in the control and experimental groups, indicators of the main energy cycles characterizing substrate-enzyme processes (pyruvic acid, lactate, cytochrome oxidase and succinate dehydrogenase), as well as antioxidant protection (reduced glutathione, glutathione reductase and glutathione peroxidase) were determined. Results. The changes recorded in the liver may reflect adaptive shifts associated with the accumulation of pyruvate as an additional substrate for mitochondrial oxidation, while in red blood cells, the accumulation of lactate contained with a decrease in the level of pyruvate indicates the formation of hypoxia. The decline of glutathione level in the liver and in erythrocytes there is a tendency to increase, which can be linked to increased transport of reduced glutathione from liver to blood. This pattern reflects the increasing role of red blood cell reduced glutathione both in regulating gas transport function and protecting cell membranes from the destructive effects of O2. Conclusion. Inhibition of glutathione peroxidase activity in essential hypercholesterolemia in both liver cells and red blood cells contributes to a viola-tion of the balance of oxidized glutathione/reduced glutathione, which accelerates atherosclerotic manifestations.

Keywords: 
hypercholesterolemia
red blood cells
liver

References: 
  1. Sudakov N.P., Novikova M.A. Strukturno-funkcional'nye narushenija mitohondrij pecheni pri ateroskleroze v jeksperimente. Izvestija Irkutskogo gosudarstvennogo universiteta. Serija «Biologija. Jekologija». 2008; 1(2): 15–19.
  2. Skulachev V.P. Programmed death phenomena: from organelletoorganism. Ann. N. Y. Acad. Sci. 2002; 959: 214–237.
  3. Mikashinovich Z.I., Belousova E.S., Semenec I.A., Romashenko A.V., Kantarija A.V. Patent na izobretenie № 2020111021 (RF). Sposob modelirovanija jessencial'noj giperholesterinemii. 16.03.2020.
  4. Spravochnik po laboratornym metodam issledovanij. Pod red. L.A. Danilovoj. SPb: Piter, 2003; s.736.
  5. Spravochnik po kliniko-biohimicheskom issledovanijam i laboratornoj diagnostike / Pod red. V.S. Kamyshnikova. M.: «MEDpress-inform», 2009; s. 896.
  6. Kulinskij V.I., Kolesnichenko L.S. Sistema glutationa. I. Sintez, transport, glutationtransferazy, glutationperoksidazy. Biomedicinskaja himija. 2009; 55(3): 255–277.
  7. Espinola-Klein C., Rupprecht H.J., Bickel C., Schnabel R., Genth-Zotz S., Torzewski M., Lackner K., Munzel T., Blankenberg S. Glutathioneperoxidase-1 activity, atheroscleroticburden, andcardiovascu-larprognosis. Am. J. Cardiol. 2007; 99(6): 808–812.