INFLUENCE 3-FORMYLCHROMONE DERIVATIVES IN VITRO AT AGGREGATION OF Β-AMYLOID PLAGUES AND TYROSINASE ACTIVITY

Objectives. Alzheimer's disease is one of the most common terminal forms of dementia, characterized by a complex pathogenesis with the for-mation of amyloid plaques in the brain structures. At the same time, one of the new and promising areas of Alzheimer's disease therapy is the influ-ence on the amyloidogenic cascade. Aim of the study. In vitro to evaluate the effect of ten 3-formylchromone derivatives on the formation of β-amyloid aggregates and tyrosinase activity. Materials and methods. The effect of the studied compounds on tyrosinase activity was evaluated using the Mapunyamethod, using L-tyrosine as a substrate and kojic acid as a reference. Aggregation of amyloid plaqueswas studied spectrophotometrically in reaction with Congo red after three and six days of incubation. Results. Among the test-objects, the most significant antithyrosinase properties were found in the 6-acetyl substituted derivative of 3-formylchromone, whose IC50 value was comparable to kojic acid (32±1.913 µg/ml versus 30.2±1.599 µg/ml). Also, this compound most significant-ly inhibited the aggregation of amyloid plaques on the third day of incubation–31.0% (p