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THE CONTENT AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OILS IN THE HERBTHYMUS MARSHALLIANUS WILLD. AND THYMUS SERPYLLUM L

DOI: https://doi.org/10.29296/25877313-2021-03-04
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Issue: 
3
Year: 
2021

A.S. Sheremetyeva Senior Lecturer, Department of General Biology, Pharmacognosy and Botany, Saratov State Medical University named after V.I. Razumovsky (Saratov, Russia) E-mail: anna-sheremetyewa@yandex.ru A.V. Frolkova Student, Saratov State Medical University named after V.I. Razumovsky (Saratov, Russia) E-mail: frolkova.anastasiya@mail.ru O.G. Shapoval Senior Lecturer, Department of Microbiology, Virology and Immunology, Saratov State Medical University named after V.I. Razumovsky (Saratov, Russia) E-mail: lavopash283741@yandex.ru N.A. Durnova Dr.Sc. (Biol.), Associate Professor, Department of General Biology, Pharmacognosy and Botany, Saratov State Medical University named after V.I. Razumovsky (Saratov, Russia) E-mail: ndurnova@mail.ru M.A. Berezutsky Dr.Sc. (Biol.), Professor, Department of General Biology, Pharmacognosy and Botany, Saratov State Medical University named after V.I. Razumovsky (Saratov, Russia) E-mail: berezutsky61@mail.ru

Essential oils of representatives of the genus thyme (Thymus L.) are characterized by a large variability in the qualitative composition and quantitative content, as well as a variety of biological activity, which determines the relevance of their study. The aim of this work is a comparative analysis of the content of essential oil in the herb Thymus marshallianus Willd. and Thymus serpyllum L. and the study of the antimicrobial activity of the vapors of their essential oils. The objects of research are the herb Thymus marshallianus Willd. and Thymus serpyllum L. The quantitative content of the essential oil was measured by steam distillation. The antimicrobial activity of essential oils was studied against standard strains of three types: Escherichia coli ATCC 25922, Staph-ylococcus aureus FDA 209P, Pseudomonas aeruginosa ATCC 27853. Statistical processing was performed using the StatSoft Statistics 10.0 software package. The content of essential oil in the herb Thymus marshallianus exceeded similar indicators in Thymus serpyllum by 3 times (1,00±0,11 and 1,81±0,19% vs. 0,22±0,07 and 0,62±0,20%) and varied depending on the climatic and geographical conditions of growth it was found. And during the study of the antimicrobial activity, it was found that both tested essential oils completely suppressed the visible growth when it was present in the control variants of Escherichia coli ATCC 25922 and Staphylococcus aureus FDA 209P strains during the entire incubation period. Pseudomonas aeruginosa ATCC 27853 showed visible growth in the presence of each of the essential oils.

Keywords: 
essential oil
Thymus marshallianus Willd.
Thymus serpyllum L.
antimicrobial activity

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