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COLLAGENOLYTIC PROPERTIES OF MICROMYCETES COLLECTION STRAINS DURING LONG-TERM STORAGE STUDY

DOI: https://doi.org/10.29296/25877313-2021-03-05
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Issue: 
3
Year: 
2021

Z.K. Nikitina Dr.Sc. (Biol.), Professor, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia) E-mail: nikitinaz@yandex.ru I.K. Gordonova Ph.D. (Biol.), Leading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia) E-mail: gordonova777@yandex.ru E.M. Nasibov Post-graduate Student, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia)

Relevance. The VILAR collection of micromycetes contains hydrolyzing collagen strains-potential producers of collagenases. When searching for en-zymes producers, the stability of viability and biosynthetic activity of microorganisms is an important indicator. In this regard, the study of colla-genolytic activity of mycelial fungi collection strains during long-term storage is relevant for solving applied and fundamental problems of modern bio-technology. Objective. Viability and collagenolytic activity of collection strains of micromycetes during long-term storage study. Material and methods. The object of the study were 16 micromycetesstrains, belonging to the genera Aspergillus, Beauveria, Paecilomyces, Penicillium, Phialophora. The cultures were stored under oil at 4 C for 4 years. After storage, the viability and collagenase activity of microorganisms were evaluat-ed during surface cultivation. In addition, deep cultivation of fungi was carried out with the determination ofcollagenolytic activity in the filtrates of the culture fluid. Results. Studies have shown that all micromycetes remained viable after 4 years of conservation under the selected conditions. During surface cultiva-tion on a modified medium with the replacement of sucrose with collagen, fungi formed colonies and well-defined lysis zones, which indicated the syn-thesis and secretion of collagenolytic enzymes. Differences in growth rates and lysis indices were found for individual species and strains of micromy-cetes. Comparison of lysis indices before and after storage showed the stability of this indicator. Using regression and correlation analysis, five strains were selected for deep cultivation. Determination of collagenolytic activity in culture fluid filtrates confirmed the synthesis and secretion of collagenolyt-ic enzymes by the studied micromycetes. Conclusions. Conservation of micromycetes on agarized Chapek media under oil at 4 C ensures the preservation of viability and collagenolytic activity. The stability of the collagenolyticproperties of micromycetes during long-term storage was confirmed. For five strains selected on the basis of regres-sion and correlation analysis, the secretion of collagenolytic enzymes into the culture medium during deep cultivation was shown.

Keywords: 
micromycetes
collagen
collagenases
surface and deep cultivation

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