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Microfluidic devices adapted for stem cell cultivation (review)

DOI: https://doi.org/10.29296/25877313-2021-11-01
Issue: 
11
Year: 
2021

E.A. Teplyashina Ph.D. (Biol.), Associate Professor, Department of Biological Chemistry with a course in medical, pharmaceutical and toxicological chemistry, Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health of the Russian Federation, (Krasnoyarsk, Russia) E-mail: elenateplyashina@mail.ru V.A. Kutyakov Ph.D. (Biol.), Associate Professor, Department of Biological Chemistry with a course in medical, pharmaceutical and toxicological chemistry, Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health of the Russian Federation (Krasnoyarsk, Russia) E-mail: victor-koutjakov@yandex.ru L.B. Shadrina Assistant, Department of Biological Chemistry with a course in medical, pharmaceutical and toxicological chemistry, Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health of the Russian Federation (Krasnoyarsk, Russia) E-mail: E-mail: shaliu@mail.ru A.B. Salmina Dr.Sc. (Med.), Professor, Brain Research Department "Scientific Center of Neurology" (Moscow, Russia) E-mail: allasalmina@mail.ru

Currently, microfluidic devices of various nature and filling are of great importance for research in the field of molecular biology, neurobiology and clini-cal medicine. Modified microfluidic analytical systems created on the basis of specialized functional elements have unique properties aimed at study-ing cellular structures and the biochemical processes occurring in them. The functional advantages of microfluidic devices include, first of all, the creation of a constant concentration gradient of reacting components, the small size of these components, the minimum consumption of reagents, the possibility of setting up high-precision experiments. Microfluidic systems also allow monitoring the state of the cellular microenvironment by simulating physiological conditions. The most promising vectors of the development of microfluidic technologies regarding the cultivation of cell cultures of various origins are analyzed. The parameters of creating 3D cellular structures are considered. The possibilities of using various microfluidic systems with respect to cell lines of various origins are investigated in order to study their functioning and identify certain patterns of development. The review summarizes the methods of culturing cell cultures of other origin using microfluidic technologies, namely: experiments related to modeling liver, kidney, tooth pulp cells, muscle or cartilage tissue
Keywords: 
microfluidic technologies
cell cultures
stem cells
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