Нажмите на эту строку чтобы перейти к Новостям сайта "Русский врач"

Перейти
на сайт
журнала
"Врач"
Перейти на сайт журнала "Медицинская сестра"
Перейти на сайт журнала "Фармация"
Перейти на сайт журнала "Молекулярная медицина"
Перейти на сайт журнала "Вопросы биологической, медицинской и фармацевтической химии"
Журнал включен в российские и международные библиотечные и реферативные базы данных

ВАК (Россия)
РИНЦ (Россия)
Эко-Вектор (Россия)

BIOPHARMACEUTICAL STUDY OF BERBERINE BISULPHATE DENTAL GEL

DOI: https://doi.org/10.29296/25877313-2022-03-02
Download full text PDF
Issue: 
3
Year: 
2022

A.G. Palvinskiy Post-graduate Student, I.M. Sechenov First Moscow State Medical University Ministry of Health of Russian Federation (Sechenov University) (Russia, Moscow) E.O. Bakhrushina Ph.D. (Pharm.), Associate Professor, I.M. Sechenov First Moscow State Medical University Ministry of Health of Russian Federation (Sechenov University) (Russia, Moscow) P.A. Kholina Student, I.M. Sechenov First Moscow State Medical University Ministry of Health of Russian Federation (Sechenov University) |(Russia, Moscow) I.I. Krasnyuk Dr.Sc. (Pharm.), Professor, I.M. Sechenov First Moscow State Medical University Ministry of Health of Russian Federation (Sechenov University (Russia, Moscow)

Abstract. The lack of uniform design requirements for the pharmaceutical development of dental gels - especially in terms of common biorelevant approaches to assessing the release of an active ingredient in vitro - complicates the harmonization of international research results with each other and slows down the entry of new effective drugs into the market. Aim. To conduct approbation of a complex of biopharmaceutical tests - diffusion into an agar layer and biodegradation in a biological fluid using a sample of berberine bisulfate dental gel. Materials and methods. In the experiment, samples of dental gels of berberine bisulfate obtained in previous studies, based on hydroxyethyl cellulose and a combination of poloxamers, were compared. Diffusion was carried out in a layer of sterile agar gel, when 1.0 g of the gel was placed in a well with a diameter of 10.0 mm, according to the size of the diameter of the colored zone with thermostating (37 ℃) for 60 minutes. Biodegradation in the oral cavity was studied on the "Dissolution" ERWEKA DT-800 tester, "Rotating paddle" apparatus (rotation speed 50 rpm) in a potassium-phosphate buffer solution pH 7.0 at 37 ± 0.1 ℃ for 1.0 g of gel in 60 minutes. Results. In the course of the studies, the advantage of using a combined gel base of poloxamers over hydroxyethyl cellulose and its effect on the rate of release of berberine bisulfate was shown. An experimental sample of a gel based on poloxamers underwent biodegradation within an hour, which, together with high rates of mucoadhesion, makes it possible to assume a long exposure of the drug when used in vivo. Conclusion. For the analyzed compositions of dental gels of berberine bisulfate, made on the basis of a combination of poloxamers and hydroxyethyl cellulose, a difference in the kinetics and completeness of release by the method of diffusion into agar was shown. Biodegradation of the dental gel of berberine bisulfate based on the combination of poloxamers was evaluated within 60 minutes and was complete. It should also be noted that the sample remained adhesive to the substrate during the first half of the test and practically did not undergo degradation.

Keywords: 
berberine bisulfate
dental gel
agar diffusion
dissolution test
poloxamers

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat или click here to download the PDF file.

References: 
  1. Duhanina I.V., Nikitina A.S., Nikitina N.V., Fes'kov S.A., Romanov V.A. Obosnovanie antibakterial'nogo dejstvija stomatologicheskih gelej na osnove Monarda Fistulosa l. jekstrakta zhidkogo. 2019; 1: DOI: https://doi.org/10.29296/25877313-2019-01-07.
  2. Novikov O.O., Zhiljakova E.T., Cimbalistov A.V., Trifonov B.V., Maljutina A.Ju., Pisarev D.I. Novyj vzgljad na problemu profilaktiki i lechenija zabolevanij parodonta. Nauchnye rezul'taty biomedicinskih issledovanij. 2016; 2(3): 6469.
  3. FDA Dissolution Methods Database. URL: http://www.oim.ru/reader.asp?nomer=366 (data obrashhenija: 23.10.2021).
  4. The United States Pharmacopeia: 〈711〉 DISSOLUTION URL: https://www.usp.org/sites/default/files/usp/document/harmonization/gen-method/stage_6_monograph_25_feb_ 2011.pdf (data obrashhenija: 23.10.2021).
  5. Masliy Yu.S., Ruban E.A. Izuchenie biofarmacevticheskih i adgezionnyh harakteristik stomatologicheskogo gelya. Vestnik farmacii. 2018; 1: 28–32. https://doi.org/10.24959/nphj.18.2188.
  6. Zagorul'ko E.Ju., Karavaeva A.S. Podhody k vyboru vspomogatel'nyh veshhestv dlja gelja stomatologicheskogo s cetilpiridinija hloridom. Farmacija i farmakologija. 2021; 9(1): 54–63. https://doi.org/10.19163/2307-9266-2021-9-1-54-63.
  7. Raszewski Z., Nowakowska-Toporowska A., Weżgowiec J., Nowakowska D. Design and characteristics of new experimental chlorhexidine dental gels with anti-staining properties. Advances in Clinical and Experimental Medicine. 2019; 28(7): 885–890. DOI: 10.17219/acem/94152.
  8. Heba A. Gad, Mohamed A. El-Nabarawi, Seham S. Abd El-Hady. Formulation and Evaluation of Secnidazole or Doxycycline Dento-Oral Gels. Drug Development and Industrial Pharmacy. 2008; 34(12): 1356–1367. https://doi.org/10.1080/03639040802122944.
  9. Alkhalidi Hala M., Khaled M. Hosny, Waleed Y. Rizg. Oral Gel Loaded by Fluconazole‒Sesame Oil Nanotransfersomes: Development, Optimization, and Assessment of Antifungal Activity. Pharmaceutics. 2021; 13(1): 27. https://doi.org/10.3390/pharmaceutics13010027.
  10. Pal'vinskij A.G., Bahrushina E.O., Kozlova Zh.M., Sinicyna A.A., Krasnjuk I.I. Razrabotka termoreversivnogo stomatologicheskogo gelja s berberinom. Razrabotka i registracija lekarstvennyh sredstv. 2020; 4(9): 88–92. https://doi.org/10.33380/2305-2066-2020-9-4-88-92.