PHARMACOLOGICAL SCREENING OF SЕRRАTULА СОRОNАTА L. HERBAL EXTRACT

DOI: https://doi.org/10.29296/25877313-2022-03-06
Issue: 
3
Year: 
2022

I.A. Lupanova Ph.D. (Biol.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia) E-mail: lupanova@vilarnii.ru O.L. Saybel Ph.D. (Pharm.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia) E.V. Ferubko Dr.Sc. (Med.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia) E.N. Kurmanova Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia) A.I. Radimitch Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia) V.K. Kolkhir Dr.Sc. (Med.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia) P.G. Mizina Dr.Sc. (Pharm.), Professor, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia)

Relevance. Natural biologically active substances are currently relevant as an important source of new herbal medicines. Phytoecdysteroids are widely accepted in medicine: they increase the non-specific body's resistance to adverse environmental factors, physical and mental strain and stress. The most accessible phytoecdysteroids sources is the Serratula Coronata aboveground part. The purpose of the study. The pharmacological screening of dry purified Serratula Coronata extract for the development of new medicines. Materials and methods. The extract was obtained by three-fold dynamic maceration of raw materials with 70% ethyl alcohol (vol.) at a temperature of (50 ± 2) oC for 60 minutes. Five phytoecdysteroids were isolated in the extract and s a new compound  20,22-propyledene 20-hydroxyecdysone. Pharmacological investigations were conducted with nonlinear white mice and nonlinear white rats. The research was approved by the VILAR Bioethical Commission. Acute toxicity was studied by the Kerber method. The Serratula Coronata extract effect on the nervous system was studied using "chloral hydrate sleep" and open field tests. The extract effect on the mice life time was studied using hypoxia with hypercapnia in the hermetic volume test. Results and discussion. It is found that that the dry purified Serratula Coronata extract is low-toxic, adaptogenic active, has a tonic effect in terms the "chloral hydrate sleep" model and antihypoxic effect in terms the "hypoxia model with hypercapnia in the hermetic volume" model., The extract does not negatively affect nervous system and experimental animals behavior. Conclusions. The dry purified Serratula Coronata extract is promising for further research to create adaptogenic herbal medicins

Keywords: 
dry purified Serratula Coronata extract
ecdysteroids
hypoxia with hypercapnia
chloral hydrate sleep model
open field model

References: 
  1. Sambukova T.V., Ovchinnikov B.V., Ganapol'skij V.P. i dr. Perspektivy ispol'zovanija fitopreparatov v sovremennoj farmakologii. Obzory po klinicheskoj farmakologii i lekarstvennoj terapii. 2017; 15(2): 56–63.
  2. Volodin V.V., Mataev S.I. Jekdisteroidsoderzhashhie rastenija  istochniki novyh adaptogenov. Vestnik biotehnologicheskoj i fiziko-himicheskoj biologii. 2011; 7(2): 52–59.
  3. Radimich A.I., Shejchenko V.I., Kuljak O.Ju., Sajbel' O.L., Korotkih I.N. Izuchenie jekdisteroidov travy serpuhi vencenosnoj (Serratula coronate L.). Voprosy obespechenija kachestva lekarstvennyh sredstv. 2020; 4 (30): 34–40.
  4. Niranjan Das, Siddhartha Kumar Mishra, Anusha Bishayee, Eunus S. Ali, Anupam Bishayee. The phytochemical, biological and medicinal attriutes of phytoecdysteroids: an updated review. Acta Pharmaceutica Sinica B. 2021; 11(7): 1740–1766. DOI: 10.1016/j.apsb.2020.10.012.
  5. Dinan L. The Karlson Lecture. Phytoecdysteroids: what use are they? Arch Insect Biochem. 2009; 72: 126–141.
  6. Jin-Jun Wang, Hao Jin, Shao-Ling Zheng, Peng Xia, Yong Cai, Xiao-Jie Ni. Phytoecdysteroids from Ajuga iva act as potential antidiabetic agent against alloxan-induced diabetic male albino rats. Biomed. Pharmacother. 2017; 96: 480–488.
  7. Cao Y., Gu C., Zhao F., Tang Y., Cui X., Shi L., Xu L., Yin L. Therapeutic effects of Cyathula officinalis Kuan and its active fraction on acute blood stasis rat model and identification constituents by HPLC-QTOF/MS/MS. Pharmacognosy Magazine. 2017; 13 (52): 693–701.
  8. Islam M.S., Choi H. Green tea, anti-diabetic or diabetogenic: a dose response study. Biofactors. 2007; 29: 45–53.
  9. Rukovodstvo po provedeniju doklinicheskih issledovanij lekarstvennyh sredstv. Chast' pervaja. M.: Grif i K. 2012; 944 s.
  10. Sernov L.N., Gacura V.V. Jelementy jeksperimental'noj farmakologii. M.: Medicina. 2000; 352 s.
  11. Lupanova I.A., Radimich A.I. Sravnitel'noe izuchenie biologicheskoj aktivnosti suhih jekstraktov serpuhi vencenosnoj Serratula Coronata L. Sbornik materialov kongressa «Chelovek i lekarstvo». Moskva. 2020; 64.
  12. Berezovskaja I.V. Klassifikacija himicheskih veshhestv po parametram ostroj toksichnosti pri parenteral'nyh sposobah vvedenija. Himiko-farmacevticheskij zhurnal. 2003; 37(3): 32–34.
  13. Kurmanova E.N., Ferubko E.V., Lupanova I.A. K farmakologii serpuhi vencenosnoj travy jekstrakta summarnogo (Serratula Coronata L.). «Molodye uchenye i farmacija XXI veka». Sbornik nauchnyh trudov tret'ej nauchno-prakticheskoj konferencii s mezhdunarodnym uchastiem. 2015; 458–460.