STUDYING OF THE ANTI-ISCHEMIC EFFECT OF OROSTACHYS SPINOSA DRY EXTRACT IN CAROTID ARTERIES BILATERAL OCCLUSION IN WHITE RATS

DOI: https://doi.org/10.29296/25877313-2022-07-01
Issue: 
7
Year: 
2022

Ya.G. Razuvaeva
Dr.Sc. (Biol.), Senior Research Scientist,
Institute of General and Experimental Biology SB RAS (Ulan-Ude, Russia)
E-mail: tatur75@mail.ru
E.A. Bayandueva
Post-graduate Student,
Banzarov Buryat State University (Ulan-Ude, Russia)
A.A. Toropova
Ph.D. (Biol.), Senior Research Scientist,
Institute of General and Experimental Biology SB RAS (Ulan-Ude, Russia)
I.G. Nikolaeva
Dr.Sc. (Pharm.), Senior Research Scientist,
Institute of General and Experimental Biology SB RAS (Ulan-Ude, Russia)

The aim of the study to evaluate the anti-ischemic effect of Orostachys spinosa dry extract in common carotid arteries bilateral occlusion in albino rats. Material and methods. The studies were carried out on 67 Wistar rats. O. spinosa extract was used as research object at doses 50, 100, 200 mg/kg. Introductory extract solution was administered intragastrically for 14 days prior to carotid arteries bilateral occlusion. The anti-ischemic effect of the O. spinosa extract was evaluated total mortality, dynamics of survival, mean life time, as well as the neurological status of animals using the modified McGraw scale and the degree of rats brain hydration. Results. It was found that the lowest animals mortality rate was observed in the experimental groups treated with O. spinosa extract at doses 100 and 200 mg/kg in carotid arteries common bilateral occlusion. The most pronounced statistically significant increase in life expectancy was observed in animals treated with O. spinosa extract at doses 50 and 100 mg/kg (by 38%, p≤0.05) arelative to that of control animals. The least pronounced neu-rological deficit degree was observed in animals treated with O. spinosa extract at doses 50 and 100 mg/kg. The most pronounced statistically signifi-cant decrease in the brain hydration level was observed in animals receiving O. spinosa extract at a dose 100 mg/kg. Life expectancy in animals, treated with dry O. spinosa extract at doses 50 and 100 mg/kg was incresed by 38% (p≤0.05) that of animals in the control group. The neurological deficit least pronounced degree was observed in ani-mals treated with O. spinosa extract at doses of 50 and 100 mg/kg. The most pronounced statistically significant decrease in the level of brain hydra-tion was observed in animals treated with dry O. spinosa extract at a dose 100 mg/kg. Conclusion. O. spinosa extract has a neuroprotective effect in cerebral ischemia, reducing the animals death percentage, increasing animals life ex-pectancy, and reducing the neurological deficit degree and the cerebral edema severity.

Keywords: 
Orostachys spinosa (L.) Sweet
anti-ischemic effect
ischemia
brain
rats

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