Нажмите на эту строку чтобы перейти к Новостям сайта "Русский врач"

Перейти
на сайт
журнала
"Врач"
Перейти на сайт журнала "Медицинская сестра"
Перейти на сайт журнала "Фармация"
Перейти на сайт журнала "Молекулярная медицина"
Перейти на сайт журнала "Вопросы биологической, медицинской и фармацевтической химии"
Журнал включен в российские и международные библиотечные и реферативные базы данных

ВАК (Россия)
РИНЦ (Россия)
Эко-Вектор (Россия)

STUDY OF CICHORIUM INTYBUS L. HERB EXTRACT HEPATOPROTECTIVE ACTIVITY IN VITRO AND IN VIVO

DOI: https://doi.org/10.29296/25877313-2022-07-04
Download full text PDF
Issue: 
7
Year: 
2022

I.A. Lupanova
Ph.D. (Biol.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia)
E-mail: lupanova@vilarnii.ru

Relevance. According to the World Health Organization recommendations it is advisable to use herbal medicines in the treatment of approximately 75% of patients with digestive system diseases. Medicinal plant raw materials contain significant amounts of biologically active substances (e.g. fla-vonoids, phenolic acids, etc.), that are of substantial interest. One of these biologically active substances sources is Cichorium intybus L. herb. The aim of research is the hepatoprotective activity comparative study of the of biologically active substances fractions different polarity of the Cicho-rium intybus L.herb extract using specific enzyme biotest systems in vitro and confirmation attained results using in vivo models. Material and methods. Common Cichorium intybus L. herb extract fractions have been under study in this research. The Silimar substance was chosen as the reference drug. Аntioxidative and antitoxic activity was studied using specific enzyme biotest-systems in vitro. The identified in vitro protective effect was manifested through the activity of enzymes marking of the morphofunctional state of hepatocytes in rats and histopathological examination, against the background of toxic hepatitis induced by one-time tetrachloromethane injection. Results. As a result of the research, it was shown that the aqueous fraction of the extract had the highest antioxidative and antitoxic activity in vitro using specific enzyme biotest-systems. Based on in vitro results it was provided the hepatoprotective activity of aqueous fraction. In vitro results were confirmed in vivo: prophylactic repeated administration of common chicory herb fractions into the body of rats with toxic liver damage caused a de-crease in the activity of AST, ALT and AP. The greatest inhibitory effect on the activity of enzymes marking the functional state of rat livers had silymar (by 15%, ALT by 33% and AP by 14%, p
Keywords: 
carbon tetrachloride treatment model
Cichorium intybus L.
chicory herb extract
fractions
hepatoprotective activity
specific enzyme biotest systems in vitro

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat или click here to download the PDF file.

References: 
  1. Dhiman R.К., Chawla Y.K. Herbal medicines for liver diseases. Digestive Diseases and Sciences. 2005; 50(10): 1807–1812.
  2. Stickel F., Schuppan D. Herbal medicine in the treatment of liver diseases. Digestive Diseases and Sciences. 2007; 39(4): 293–304.
  3. Ivashkin V.T., Baranovskij A.Ju., Rajhel'son K.L. i dr. Lekarstvennye porazhenija pecheni (klinicheskie rekomendacii dlja vrachej). Rossijskij zhurnal gastrojenterologii, gepatologii, koloproktologii. 2019; 29(1):85–115.
  4. Levy C., Seeff L.D., Lindor K.D. Use of herbal supple-ments for chronic liver disease. Clinical Gastroenterology and Hepatology. 2004; 2(11):947–956.
  5. Lam P., Cheung F., Tan H.Y. Hepatoprotective Effects of Chinese Medicinal Herbs: A Focus on Anti-Inflammatory and Anti-Oxidative Activities. International Journal of Molecular Sciences. 2016; 17(4):465.
  6. Rino Y., Yukawa N., Yamamoto N. Does herbal medicine reduce the risk of hepatocellular carcinoma? World Journal of Gastroenterology. 2015; 21(37):10598–10603.
  7. Verma S., Thuluvath P.J. Complementary and alternative me-dicine in hepatology: review of the evidence of efficacy. Cli-nical Gastroenterology and Hepatology. 2007; 5(4):408–416.
  8. Mizina P.G. Rastitel'nye i mineral'nye biologicheski aktivnye kompleksy dlja medicinskih tehnologij zdorov'esberezhenija. M. Nauka, 2021. 164 s.
  9. Global'naja strategija sektora zdravoohranenija po virusnomu gepatitu 2016–2021. Vsemirnaja organizacija zdravoohranenija. Zheneva, 2016. [Jelektronnyj resurs].URL: http://www.who.int/hepatitis/strategy2016-2021/ghss-hep/ru/.
  10. Liu Y., Flynn T.J., Ferguson M.S., Hoagland E.M. Use of the Combination. Index to determine interactions between plant-derived phenolic acids on hepatotoxicity endpoints in human and rat hepatoma cells. Phytomedicine. 2013; 20:461–468.
  11. Karthikesan K., Pari, L., Menon V.P. Antihyperlipidemic effect of chlorogenic acid and tetrahydrocurcumin in rats subjected to diabetogenic agents. Chemico-Biological Interactions. 2010; 188: 643–650.
  12. Karthikesan K., Pari L., Menon V.P. Combined treatment of tetrahydrocurcumin and chlorogenic acid exerts potential anti-hyperglycemic effect on streptozotocin-nicotinamide-induced di-abetic rats. General Physiology and Biophysics. 2010; 29: 23–30.
  13. Prabhakar P.K., Doble M. Synergistic effect of phytoche-micals in combination with hypoglycemic drugs on glu-cose uptake in myotubes. Phytomedicine. 2009;16: 1119–1126.
  14. Yahfoufi N., Alsadi N., Jambi M. et al. The Immunomodulatory and Anti-Inflammatory Role of Polyphenols. Nutrients. 2018; 10(11):1618.
  15. Fernandez-Panchon M.S, Villano D., Troncoso A.M. Antioxidant activity of phenolic compounds: from in vitro results to in vivo evidence. Critical Reviews in Food Science and Nutrition. 2008; 48(7):649–671.
  16. Righi N., Boumerfeg S., Deghima A. et al. Phenolic profile, safety assessment, and anti-inflammatory activity of Salvia verbenaca L. Journal of Ethnopharmacology. 2021; 272: 113940.
  17. Sajbel' O.L., Radimich A.I., Adamov G.V. i dr. Sravnitel'noe fitohimicheskoe izuchenie nadzemnoj chasti dikorastushhego i kul'tiviruemogo rastenija cikorija obyknovennogo (Sichorium intybus L.). Himija rastitel'nogo syr'ja. 2020; 3:187–195.
  18. Elgengaihi S., Mossa А-T.H, Refaie A.A. Hepatoprotective Efficacy of Cichorium intybus L. Extract Against Carbon Tetrachloride-induced Liver Damage in Rats. Journal of Dietary Supplements. 2016; 13(5):570–584.
  19. Sajbel' O.L., Radimich A.I., Dargaeva T.D. i dr. Patent RU 2771028. Sposob poluchenija sredstva, obladajushhego gepatoprotektornym i anti-gepatotoksicheskim dejstviem. Opubl. 25.04.2022.
  20. Sajbel' O.L., Radimich A I., Dargaeva T.D. i dr. Fenol'nye soedinenija i gepatoprotektornaja aktivnost' jekstrakta travy cikorija obyknovennogo. Razrabotka i registracija lekarstvennyh sredstv. 2021; 10(4):36–45.
  21. Vichkanova S.A., Kolhir V.K., Sokol'skaja T.A. i dr. Lekarstvennye sredstva iz rastenij (opyt VILAR): nauchnoe izdanie. M. ADRIS. 2009. 432 s.
  22. Beuter E. Red cell enzyme defects. Hematologic Pathology. 1990; 4(3): 103–114.
  23. Koroljuk M.A., Ivanova M.I., Majorova I.G. i dr. Metod opredelenija aktivnosti katalazy. Laboratornoe delo. M.: Medicina. 1988; 1:1–8.
  24. Habig W.H., Pabst M.J., Jakoby W.B. Glutathione S-Transferases: the first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry. 1974; 249(22):7130–7139.
  25. Zhukov A.A., Archakov A.I. Stehiometrija reakcij mikrosomal'nogo okislenija. Raspredelenie redoks-jekvivalen-tov mezhdu monooksigenaznymi i oksidaznymi reakcijami, kataliziruemymi citohromom R 450. Biohimija. 1985; 50(12):1939–1952.
  26. Omura T., Sato R. The carbon monoxide-binding pigment. Journal Biological Chemistry. 1964; 7: 2370–2378. https://doi.org/10.1016/S0021-9258(20)82245-5.
  27. Lowry O.H., Rosenberg N.Y., Farr A.J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1): 265–275.
  28. Karuzina I.I., Bachmanova G.I., Mengazetdinov D.Je., Mjasoedova K.N., Zhihareva V.O., Kuznecova G.I., Archakov A.I. Vydelenie i svojstva citohroma R450 iz mikrosom pecheni krolikov. Biohimija. 1979; 6: 1049–1057.
  29. Rukovodstvo po provedeniju doklinicheskih issledovanij lekarstvennyh sredstv. Chast' pervaja. Pod red. A.N. Mironova. M.: Grif i K, 2012. 944 s.
  30. Gutteridge J.M., Halliwell B. Free radicals and antioxidants in the year 2000. A historical look to the future. Ann. NY Acad. Sci. 2000; 899:136–147.
  31. Conwell D.G., Jones K.H., Jiang Z., Lantry L.E., Keely P.S.W., Kohar I., Thornton D.E. Cytotoxicity of tocopherols and their quinines in drug-sensitive and multidrug-resistant leukemia cells. Lipids. 1998; 33:295–301.
  32. Cook N.C., Samman S. Flavonoids chemistry, metabolism, cardioprotective effects, and dietary sources. J. Nutr. Bio-chem. 1996; 7:66–76.
  33. Middleton E.J., Kandaswami C. Effects of flavonoids on im-mune and inflammatory cell functions. Biochem Pharmacol. 1992; 43:1167–1179.
  34. Roger C.R. The nutritional incidence of flavonoids: some physiological and metabolic considerations. Experientia 1988; 44:725–733.
  35. Bykov V.A., Dubinskaja V.A., Mineeva M.F. i dr. Patent RU № 2181892. Sposob vyjavlenija veshhestv, obladajushhih antioksidantnymi svojstvami, in vitro. Opubl. 27.04.2002.
  36. Pouille C.L., Ouaza S., Roels E. et al. Chicory: Under-standing the Effects and Effectors of This Functional Food. Nutrients. 2022; 14(5):957.
  37. Epure A., Pârvu A.E., Vlase L. et al. Phytochemical profile, antioxidant, cardioprotective and nephroprotective activity of romanian chicory extract. Plants. 2021; 10:64.
  38. Jasim R.S. Antioxidant, antimicrobial activities and phytoche-mical constituents of Cichorium intybus L. Aerial Parts. Int. J. Bot. 2018; 14:24–29.
  39. Lukashina T.V., Mineeva M.F., Dubinskaja V.A. i dr. Ispol'zovanie specificheskih fermentnyh biotest-sistem in vitro dlja razrabotki mnogokomponentnogo preparata Stabinorm. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2007; 4: 25–30.
  40. Ahmed B., Al-Howiriny T.A., Siddiqui A.B. Antihepatotoxic activity of seeds of Cichorium intybus. J. Ethnopharmacol. 2003; 87(2–3):237–240.
  41. Buko V., Zavodnik I., Budryn G. et al. Chlorogenic Acid Protects against Advanced Alcoholic Steatohepatitis in Rats via Modulation of Redox Homeostasis, Inflammation, and Lipogenesis. Nutrients. 2021; 13(11):4155.