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EFFECT OF MODULATION OF NITROGEN OXIDE (II) SYNTHESIS UNDER CHRONIC NORMOBARIC HYPOXIA ON THE ISOENZYME SPECTRUM OF RAT EPIDIDIMIS LACTATE DEHYDROGENASE

DOI: https://doi.org/10.29296/25877313-2023-01-09
Issue: 
1
Year: 
2023

Y.A. Marsyanova
Assistant, Department of Biological Chemistry with the Course of CLD FAPE,
Ryazan State Medical University named after academician I.P. Pavlov (Ryazan, Russia)
E-mail: yuliyamarsyanova@yahoo.com; ORCID: 0000-0003-4948-4504, SPIN-код: 4075-3169
V.I. Zvyagina
Ph.D., Associate Professor, Department of Biological Chemistry with the Course of CLD FAPE,
Ryazan State Medical University named after Academician I.P. Pavlov (Ryazan, Russia)
E-mail: vizvyagina@yandex.ru
D.A. Solovykh
Student,
Ryazan State Medical University named after Academician I.P. Pavlov (Ryazan, Russia)
E-mail: solovykh1234@mail.ru

Lactate dehydrogenase have a key role in providing energy to cells under physiological and hypoxic conditions. Changes in the activity of the enzyme can be facilitated by nitric oxide (II), regulating the expression of individual fractions of lactate dehydrogenase. Aim. To study changes in the isoenzyme spectrum of rat epididymis lactate dehydrogenase under conditions of hypoxia and modulation of nitric oxide (II) synthesis. Material and methods. Male rats (32) were divided into 4 groups (n=8): 1) chronic normobaric hypoxia; 2) control to group 1; 3) hypoxia together with modeling of nitric oxide deficiency; 4) hypoxia against the background of induction of NO synthesis. The mitochondrial fraction and non-mitochondrial cytoplasm of the head and tail of the epididymis were isolated for laboratory research. The zymogram was obtained by electrophoresis in 7% gel followed by detection by the precipitation reaction of the reduction of nitro blue tetrazolium. The overall activity of lactate dehydrogenase was assessed and the percentage of isoenzyme fractions was calculated. Fractions 1 and X of lactate dehydrogenase were analyzed, the results were con-sidered statistically significant if p
Keywords: 
chronic hypoxia
lactate dehydrogenase
LDH
electrophoresis
epididymis.
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