Нажмите на эту строку чтобы перейти к Новостям сайта "Русский врач"

Перейти
на сайт
журнала
"Врач"
Перейти на сайт журнала "Медицинская сестра"
Перейти на сайт журнала "Фармация"
Перейти на сайт журнала "Молекулярная медицина"
Перейти на сайт журнала "Вопросы биологической, медицинской и фармацевтической химии"
Журнал включен в российские и международные библиотечные и реферативные базы данных

ВАК (Россия)
РИНЦ (Россия)
Эко-Вектор (Россия)

DEVELOPMENT AND VALIDATION OF THE QUANTITATIVE DETERMINATION METHOD FOR A NEW BIOLOGICALLY ACTIVE COMPOUND N-[2-[4-OXO-3(4Н)-QINAZOLINYL]PROPIONYL]-GUANIDINE BY THE METHOD OF TITRATION IN A NON-AQUEOUS MEDIUM

DOI: https://doi.org/10.29296/25877313-2023-09-05
Issue: 
9
Year: 
2023

D.N. Lutsenko
Specialist in Educational and Methodological Work, Department of Biological Chemistry,
Pyatigorsk Medical and Pharmaceutical Institute – Branch of the State Budgetary Educational Institution of Higher Education
"Volgograd State Medical University" of the Ministry of Health of the Russia (Pyatigorsk, Russia)
E-mail: lucenkodasha95@mail.ru
E.V. Kompantseva
Dr.Sc. (Pharm.), Professor, Department of Pharmaceutical Chemistry,
Pyatigorsk Medical and Pharmaceutical Institute – a Branch of the State Budgetary Educational Institution
of Higher Education "Volgograd State Medical University" of the Ministry of Health of the Russia (Pyatigorsk, Russia)
E-mail: dskompanceva@mail.ru
A.S. Chiriapkin
Lecturer, Department of Pharmaceutical Chemistry, Pyatigorsk Medical and Pharmaceutical Institute –
Branch of the State Budgetary Educational Institution of Higher Education "Volgograd State Medical University"
of the Ministry of Health of the Russia (Pyatigorsk, Russia)
E-mail: alexey.chiriapkin@yandex.ru -mail,
L.S. Ushakova
Ph.D.(Pharm.), Associate Professor, Department of Toxicological and Analytical Chemistry,
Pyatigorsk Medical and Pharmaceutical Institute – a Branch of the State Budgetary Educational Institution
of Higher Education "Volgograd State Medical University" of the Ministry of Health of the Russia (Pyatigorsk, Russia)
E-mail: uchakova2002@yandex.ru

Relevance. Due to the wide spread of diseases of the cardiovascular system, the development and study of drugs aimed at the treatment of diseases of the circulatory system is relevant. A new biologically active compound (BAC) is synthesized at the Department of Pharmaceutical and Toxicological Chemistry of the Volgograd State Medical University – a derivative of quinazoline: N-[2-[4-oxo-3(4H)-quinazolinyl] propionyl]-guanidine (VMA-13-15), which at the stage of preclinical studies confirmed the prospects for use in medical practice as a cardioprotective and neuroprotective drug. The aim of the study is to develop and validate a method for the quantitative determination of N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]-guanidine (VMA-13-15). Material and methods. The objects of study are laboratory samples of N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]-guanidine (VMA-13-15, series 2019, 2020 and 2021). A laboratory sample VMA-13-15 twice recrystallized from methanol and dried to constant weight is used as a standard sample (RS). Quantitative determination is carried out by acid-base titration in a non-aqueous medium. The end of the titration is fixed potentiometrically. Results. The ionization constants VMA-13-15 are determined. A procedure is developed for the quantitative determination of N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]guanidine by acid-base titration in a solvent medium of glacial acetic acid with the establishment of an equivalence point using a potentiometer. The developed method is validated according to the main characteristics: specificity, linearity, correctness, convergence and analytical area. The validation analysis of the developed method confirms that it is suitable for the quantitative determination of the main substance content in the VMA-13-15 substance. Conclusions. A technique for potentiometric titration of N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]guanidine in a non-aqueous medium is developed and validated.
Keywords: 
N-[2-[4-oxo-3(4H)-quinazolinyl]propionyl]guanidine
ionization constants
potentiometric determination
validation.
References: 
  1. Patent RU 2 622638 C1. Proizvodnye hinazolin-4(3N)-ona, obladajushhie nejro- i kardioprotektornoj aktivnost'ju. V.I. Petrov, I.N. Tjurenkov,
  2. A.A. Ozerov, M.S. Novikov i dr. Opublikovanno: 19.06.17. Bjul.17.
  3. Gluhova E.G. Sintez i farmakologicheskie svojstva novyh karbonil'nyh proizvodnyh hinazolin-4(3N)-ona: Diss. … kand. farm. nauk. Volgograd. 2016;
  4. 120 s.
  5. Zijatdinova G.K., Varlamova R.M., Medjanceva Je.P. Titrimetricheskie metody v farmacevticheskom analize: uchebnoe posobie [Jelektronnyj resurs]. Kazan': Kazan. un-t, 2018: 98 s.
  6. Kompanceva E.V., Lucenko D.N., Garsija E.R. Opredelenie biologicheski aktivnogo soedinenija N-[2-[4-okso-3(4N)-hinazolinil]propionil]-guanidina metodom kapilljarnogo jelektroforeza Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2020; 23(10): 40–45.
  7. Al'bert A., Serzhent E. Konstanty ionizacii kislot i osnovanij. Moskva, Leningrad: Himija, 1964; 380 c.
  8. Kompanceva E.V, Lucenko D.N., Glushko A.A. Razrabotka i validacija metodiki kolichestvennogo opredelenija biologicheski aktivnogo soedinenija n-(2-[4-okso-3(4H)-hina-zolinil] propionil)guanidina. Aspirantskij vestnik Povolzh'ja. 2019; 56: 122–127.
  9. ChemAxon – Software Solutions and Services for Chemistry and Bioloogy. [Jelektronnyj resurs]. Rezhim dostupa: https://chemicalize.com.
  10. Gosudarstvennaja farmakopeja Rossijskoj Federacii. XIV izdanie. MZ RF. M., 2018; T. 1. URL: http:// femb.ru/femb/ pharmacopea.php (data obrashhenija 22.09.2022 g.).
  11. Daksh S., Goyal A., Pandiya C.K. Validation of analytical methods - Strategies &Singficance. Int. J. Res. Dev. Pharm. L. Sci. 2015; 4(3): 489–497.