DEVELOPMENT OF QUALITY INDICATORS OF TRICHODERMA ATROBRUNNEUM CULTURE FLUID EXTRACT VKPM F-1434

Relevance. It is relevant to solve the problems of quality control of medicinal substances, drugs, preparations by conducting pharmaceutical analy-sis. The purpose of the study – determination of physico-chemical and biological properties of Trichoderma atrobrunneum VKPM F-1434 culture fluid ex-tract. Development of a method for determining the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 in bioproducts. Material and methods. The strain Trichoderma atrobrunneum VKPM F-1434 synthesizing low molecular weight biologically active compounds with bacteriostatic effect was used in the work. The extract was obtained by single-stage mixing and settling extraction with ethyl acetate from the culture liquid of T. atrobrunneum VKPM F-1434 after 5 days of deep cultivation at a temperature of 28 ° C, the seed material was the vegetative mycelium of the producer in the late exponential growth phase. The mass concentration of T. atrobrunneum VKPM F-1434 culture fluid extract was determined in a healing liniment for wound healing. Results. Based on the study of physico-chemical and biological properties of culture fluid extract T. atrobrunneum VKPM F-1434, its individual charac-teristics have been established. The method "Determination of the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 in the fin-ished bioproduct" has been developed. Scope of the method: determination of the extract’s mass concentration at the stages of the technological pro-cess of preparation, as well as in ready-made dosage forms, therapeutic and preventive orientation of zootechnical and veterinary purposes. Character-istic of the method: the range of determined values – mass concentrtion (ω): 1 – 10%; repeatability: CV = 1.69%; reproducibility CV1 = 1.68%; CV2 = 1.78%; Fr = 1.09; Ft = 3.44; correctness: R = 100.1%. Conclusions. Physico-chemical and biological properties of culture fluid extract T. atrobrunneum VKPM F-1434 can be used to establish its authenticity and quality assessment. The method of determining the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 can be considered suitable for a reliable assessment of the composition of the finished bioproduct.