EXTRACTION OF NUCLEIC ACIDS FROM THE CELL BIOMASS OF THE MICROALGAE CHLORELLA VULGARIS AND DETERMINATION OF THEIR AMOUNT BY PHOTOCOLORIMETRIC METHOD

DOI: https://doi.org/10.29296/25877313-2020-01-06
Issue: 
1
Year: 
2020

B.O. Roik Post-graduate Student, Department of Physiology and Chemistry name prof. A.A. Sisoev, Kursk SAA name I.I. Ivanov E-mail: bogdan.biochem.roik@mail.ru M.M. Naumov Dr.Sc. (Veterinary), Professor, Department of Physiology and Chemistry name prof. A.A. Sisoev, Kursk SAA name I.I. Ivanov E-mail: naumovmm@rambler.ru

The paper presents the results of quantitative analysis of nucleic acids in the green microalgae Chlorella vulgaris (IGF strain no. C-111). The nu-cleic acids present in the Chlorella cell are involved in the transmission of encoded genetic information from generation to generation of microalgae, ensuring the safety and longevity of the species. Information about the amount of nucleic acids present in Chlorella, will further create drugs based on DNA and RNA in the form of sodium salts. Alkaline and acidic hydrolysis of RNA and DNA from the biomass of the microorganism was carried out ac-cording to the adapted and adapted biochemical method of Schmidt-Thanhauser. An effective method of photocolorimetric determination of the amount of DNA and RNA in the hydrolysate from specific color reactions to carbohydrate monomers Deoxyribose and Ribose, respectively, using a 96-well plate photometer-reader used for enzyme immunoassay (ELISA) screening was developed and modernized. Was determined empirically optimal filter at 620 nm (orange) for check painted joints desoxyribose and ribose after color reactions specific solutions of reagents of the diphenylamine and orcine. Appropriate calibration dependences with a linearity coefficient of at least r = 0.999 were constructed for the quantitative determination of DNA and RNA in the volume of hydrolysates, which made it possible to determine the amount of nucleic acids in the sample of Chlorella vulgaris biomass. This method of colorimetric analysis provides a sufficiently high accuracy in quality control of preparations based on nucleic acids in analytical studies in the laboratory.

Keywords: 
desoxyribose
DNA and RNA
method of Schmidt-Thanhauser
microalgaes
nucleic acids
ribose
photocolorimetry method
Chlorella vulgaris

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