COLLECTION OF CELL CULTURES AS AN ALTERNATIVE SOURCE OF BIOLOGICALLY ACTIVE SUBSTANCES

VILAR is the holder of a collection of 16 strains of cultures of nine medicinal plants species: Macleaya cordata Willd, Thalictrum minus L., Stephania gla-bra Roxb., Miers, Podophyllum peltatum L., Panax ginseng C. A.Mey, Rodiola rosea L., Macrotomia eychroma Royle Pauls, Ungernia victoris Vved.ex Artjuschenko, Hedysarum neglectum. The presented cultures contain a wide range of biologically active substances: alkaloids (berberine, sanguinarine, helerubin, stefarin, galantamine); saponins, phenylpropanoids, lignans (podophyllotoxin), glycosides (mangiferin), shikonin. During cultivation, cell strains are maintained in the form of a suspension culture in a nutrient medium according to the Murasige-Skoog prescriptions with the addition of the necessary vitamins and growth regulators and in the form of callus on agar. The viability of the crops is maintained on a hang-ing rocking chair with a frequency of 100 rpm in a dark room at a temperature of 26º C and in thermostats TC80M2. To overcome the instability of the processes of growth and synthesis of target secondary metabolites during long-term cultivation, cell strains are maintained under strictly controlled conditions in accordance with the cultivation technology - periodic transplants and regular monitoring of the content of the main active substances. Qualitative and quantitative analysis of the target components is carried out by a chromatospectrophotometric method. We find, that thalictrum minus biomass productivity and berberine biosynthesis (up to 0.10–0.12%) are kept a long time (more than 13 years). The maximum synthesis of alkaloids sanguinarine and helerubine (up to 0.88%) in total cell culture of macleaya is kept more than 8 years. This confirms the sufficient stability of the obtained strains. There was also find the variation in the level of maximum accumulation of target metabolites in the pro-cess of cultivation during the year with the maximum level at spring -sampler season.