STANDARDIZATION OF ANTI-INFLAMMATORY HERB TEA

DOI: https://doi.org/10.29296/25877313-2022-05-05
Issue: 
5
Year: 
2022

P.B. Lubsandorzhieva
Dr.Sc. (Pharm.), Laboratory of Biomedical Research,
Institute of General and Experimental Biology of the SB RAS (Ulan-Ude, Russia)
E-mail: bpunsic@mail.ru
N.I. Kashenko
Dr.Sc. (Pharm.), Laboratory of Biomedical Research,
Institute of General and Experimental Biology of the SB RAS (Ulan-Ude, Russia)
T.D. Dargaeva
Dr.Sc. (Pharm.), Professor, Department of Standardization and Certification,
All-Russian Scientific Research of Medicinal and Aromatic Plants (Moscow, Russia)

The composition of anti-inflammatory herbal tea intended for the treatment and prevention of chronic colitis includes Bergenia crassifolia (L.) Fritsch. black leaves, Matricaria chamomilla L. flowers, Achillea millefolium L. herb and Mentha piperita L. leaves. Standardization of anti-inflammatory herbal tea is the purpose of this work. Material and methods. Samples of herbal tea produced by SMEs "Arura" (Ulan-Ude) were used. The microcolumn HPLC method was used to inves-tigate the composition of phenolics. The total contents of biologically active substances were determined according to pharmacopoeial methods. Results. The contents of arbutin, gallic acid, bergenin, chlorogenic acid, rutin, rosmarinic acid in herbal tea were 1.10, 0.12, 0.34, 0.05, 0.46 %, re-spectively. The total contents of flavonoids, phenolic acids, tannins, triterpenes, anthocyanins, organic acids, water-soluble polysaccharides, pectin substances, amino acids in herbal tea were 2.96, 0.21, 9.45, 1.24, 0.21, 3.90, 4.10, 0.90, 0.14%, respectively. A differential method for the quantita-tive determination of flavonoids was adapted to standardize the herbal tea, the error of a single determination of the content of flavonoids did not ex-ceed ±4.45%. Diagnostically significant microscopic features of herbal tea were established. Minimal content levels of the main groups of biologically active substances in anti-inflammatory herbal tea have been established. Thus, total contents of flavonoids (luteolin equivalents), tannins and arbutin were not less than 2.0, 8.0, 0.5%, respectively. Conclusions. Anti-inflammatory herbal tea is proposed to standardize according to the sum of phenolics: flavonoids (luteolin equivalents), tannins and arbutin. Minimal content levels of the main phenolics, diagnostically significant microscopic features of herbal tea were established.

Keywords: 
Key words: anti-inflammatory herbal tea
phenolic compounds
methods of standardization.

References: 
  1. Azhunova T.A., Lubsandorzhieva P.B. Farmakoterapevticheskaja jeffektivnost' kompleksnogo rastitel'nogo sredstva pri jeksperimental'nom kolite i disbakterioze. Vestnik Burjatskogo gosudarstvennogo universiteta. 2021; 2: 21–26.
  2. Lubsandorzhieva P.B., Boldanova N.B. Letuchie komponenty protivovospalitel'nogo sbora. Rastitel'nye. resursy. 2012; 48(3): 396–402.
  3. Ke F., Yadav P.I., Ju L.Z. Herbal medicine in the treatment of ulcerative colitis. Saudi J Gastroenterol. 2012; 18(1): 3–10.
  4. Shikov A.N., Pozharitskaya O.N., Makarova M.N. et al. Bergenia crassifolia (L.) Fritsch – pharmacology and phytochemistry. Phytomedicine. 2014; 21:
  5. 1534–1542.
  6. Murti K. Pharmacological properties of Matricaria recutita: a review. Pharmacologia. 2012; 3: 348–351.
  7. Mohammadhosseini M., Sarker S.D., Akbarzadeh A. Chemical composition of the essential oils and extracts of Achillea species and their biological activities:
  8. A review. J Ethnopharm. 2017; 199: 257–315.
  9. Sharifi-Rad M., Nazaruk J. Polito L. et al. Matricaria genus as a source of antimicrobial agents: from farm to pharmacy and food applications. Microbial Research. 2018; 215: 76–88.
  10. Tyagi A.K., Malik A. Antimicrobial potential and chemical composition of Mentha piperita oil in liquid and vapour phase against food spoiling microorganisms. Food Control. 2011; 22: 1707–1714.
  11. Lemmens-Gruber R., Marchart E., Rawnduzi P., et al. Investigation of the spasmolitic activity of the flavonoid fraction of Achillea millefolium s.l. on isolated quinea-pig itea. Arzneim.-Forsch. 2006; 56: 582–588.
  12. Cholamipourfard K., Salehi M., Banchio E. Mentha piperita phytochemicals in agriculture, food industry and medicine: features and applications. South African Journal of Botany. 2021; 141: 183–195.